ndh [2019-06-27 15:32:31]

NADH dehydrogenase (Menaquinone 7 & no proton)

locus: BSU12290

pI: 6.29

mw: 41.79 kDa

protein length: 392 aa

gene length: 1179 bp

function: respiration

product: NADH dehydrogenase (Menaquinone 7 & no proton)

essential: no

synonyms: yjlD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.4|Respiration/ other]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,299,074 1,300,252

Phenotypes of a mutant

• inactivation of ''ndh'' facilitates without a cell wall (due to reduction of oxidative stress) [Pubmed|26051891]
• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

The protein

Protein family

• NADH dehydrogenase family (with [protein|5044FDBEC8D81D1978DB431F9222902FCD80DD2D|YutJ] and [protein|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|YumB], according to UniProt)

Paralogous protein(s)

• [protein|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|YumB]

[SW|Cofactors]

• FAD [pubmed|31133996]

Structure

• [PDB|4NWZ] (from ''C. thermarum'', 42% identity) [Pubmed|24444429]

[SW|Localization]

• membrane associated [Pubmed|18763711]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|8279630BE1D1D214162BA8E06FC05146F9ED517D|yjlC]-[gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]
  description: [Pubmed|17015645]

  regulatory mechanism

  • [protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]: repression, [Pubmed|17015645], in [regulon|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: repression, [pubmed|28439033], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

  regulation

  • induced at high NADH⁺ levels ([protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]) [Pubmed|17015645]
  • repressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [pubmed|28439033,10913079]

• genes: [gene|2F5683B654BD1B7FA4D55011CCE6413C38DB3F4D|yjlB]-[gene|8279630BE1D1D214162BA8E06FC05146F9ED517D|yjlC]-[gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A358 (yjlD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/358 NBRP B. subtilis, Japan]
• BKE12290 ([gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTATATCCTCCGTCCT, downstream forward: _UP4_TAATCCTTTTAATGAATCTG
• BKK12290 ([gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTATATCCTCCGTCCT, downstream forward: _UP4_TAATCCTTTTAATGAATCTG

References

• 17015645,20933603,11948165,18763711,15378759,26051891,24444429,28439033,10913079,28189581,31133996